Here, we provide a demonstration of the suction blister cutaneous recall model. The model allows a simple access to study human in vivo adaptive immune responses, for instance in the context of vaccine development.
Holm, L. L., Vukmanovic-Stejic, M., Blauenfeldt, T., Benfield, T., Andersen, P., Akbar, A. N., Ruhwald, M. A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo. J. Vis. Exp. (138), e57554, doi:10.3791/57554 (2018).