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Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs
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June 01, 2018
DOI:
1Department of Stem Cell Biology and Regenerative Medicine,Keck School of Medicine of the University of Southern California, 2Caruso Department of Otolaryngology-Head and Neck Surgery,Keck School of Medicine of the University of Southern California, 3Howard Hughes Medical Institute and Laboratory of Sensory Neuroscience,The Rockefeller University
Summary
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Three-dimensional organotypic cultures of the murine utricle and cochlea in optically clear collagen I gels preserve innate tissue morphology, allow for mechanical stimulation through adjustment of matrix stiffness, and permit virus-mediated gene delivery.
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Gnedeva, K., Hudspeth, A. J., Segil, N. Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs. J. Vis. Exp. (136), e57527, doi:10.3791/57527 (2018).
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