/
/
Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs
8,086 Views
•
08:17 min
•
June 01, 2018
DOI:
1Department of Stem Cell Biology and Regenerative Medicine,Keck School of Medicine of the University of Southern California, 2Caruso Department of Otolaryngology-Head and Neck Surgery,Keck School of Medicine of the University of Southern California, 3Howard Hughes Medical Institute and Laboratory of Sensory Neuroscience,The Rockefeller University
Summary
Automatically generated
Three-dimensional organotypic cultures of the murine utricle and cochlea in optically clear collagen I gels preserve innate tissue morphology, allow for mechanical stimulation through adjustment of matrix stiffness, and permit virus-mediated gene delivery.
Read Article
Cite this Article
Gnedeva, K., Hudspeth, A. J., Segil, N. Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs. J. Vis. Exp. (136), e57527, doi:10.3791/57527 (2018).
Copy