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The Lambda Select cII Mutation Detection System
Chapters
- 00:04Title
- 00:52In Vitro Packaging Reaction
- 01:51Plating the Packaged DNA Samples
- 03:36Examination of the Titer and the Screening Plates to Determine the Putative cll Mutants, Verification of Mutant Plaques, PCR Amplification, and DNA Sequencing
- 05:31Results: Use of λ Select cll Assay to Detect Mutations in the cll Transgenes from the Genomic DNA
- 06:31Conclusion
We describe a detailed protocol for the Lambda Select cII mutation assay in cultured cells of transgenic rodents or the corresponding animals treated with a chemical/physical agent of interest. This approach has been widely used for mutagenicity testing of carcinogens in mammalian cells.
