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Genetics

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JoVE Journal Genetics
Enhanced Genome Editing con Cas9 ribonucleoproteina nelle Diverse cellule e degli organismi
 
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Enhanced Genome Editing con Cas9 ribonucleoproteina nelle Diverse cellule e degli organismi

Article DOI: 10.3791/57350-v • 09:51 min • May 25th, 2018
May 25th, 2018 •
*1,2, *1, *3,4,5,6, *1,3, 1,3, 3,5,6,7,8,9, 1,2, 1,10, 3
1Department of Molecular Cell Biology, University of California, Berkeley, 2Howard Hughes Medical Institute, University of California, Berkeley, 3Innovative Genomics Institute, University of California, Berkeley, 4Biomedical Sciences Graduate Program, University of California, San Francisco, 5Department of Microbiology and Immunology, University of California, San Francisco, 6Diabetes Center, University of California, San Francisco, 7Chan Zuckerberg Biohub, 8Department of Medicine, University of California, San Francisco, 9UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, 10Department of Integrative Biology, University of California, Berkeley
* These authors contributed equally

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요약
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Utilizzando un Cas9 preassemblati complesso ribonucleoproteico (RNP) è un metodo potente per l'editing genomico precisa, efficiente. Qui, si evidenzia sua utilità in una vasta gamma delle cellule e degli organismi, tra cui cellule umane primarie e classico ed emergenti organismi modello.

Tags

Genetica problema 135 CRISPR Cas9 gRNA RNP editing genomico gene editing off-target HDR HSPCs cellule T elegans di Caenorhabditis Parhyale hawaiensis
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