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Journal / Genetics / A Method to Study the C924T Polymorphism of the…
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Genetics

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JoVE Journal Genetics
A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene
 

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

Article DOI: 10.3791/57289-v • 07:00 min • April 1st, 2019
April 1st, 2019 •
1, 1, 2, 2, 2, 1, 3, 1, 2, 4, 1,2
1SS Annunziata University Hospital, Unit of Clinical Molecular Biology and Predictive Medicine, University of Chieti, 2Department of Medical, Oral and Biotechnological Sciences, University of Chieti, 3Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, 4CNR - Institute of Molecular Genetics, Section of Chieti

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In the present study, we describe a methodology to analyze the C924T genotype. The protocol consists of three phases: DNA extraction, amplification by polymerase chain reaction (PCR), and analysis of the restriction fragment length polymorphism (RFLP) on agarose gel.

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C924T Polymorphism Thromboxane A2 Receptor Gene Method Study Thrombosis Hemostasis Atherothrombotic Processes High-risk Patients Aspirin Inexpensive Technique Simple To Use Convenient High Efficiency Rapid Identification Polymorphisms Personalized Medicine Specific Drugs Pharmacological Individual Response Tris EDTA Buffer Electrophoresis Buffer Gel Loading Dye 1X TBE Buffer Agarose
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