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A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene
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April 01, 2019
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1SS Annunziata University Hospital, Unit of Clinical Molecular Biology and Predictive Medicine,University of Chieti, 2Department of Medical, Oral and Biotechnological Sciences,University of Chieti, 3Department of Biotechnological and Applied Clinical Sciences,University of L’Aquila, 4CNR – Institute of Molecular Genetics, Section of Chieti
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In the present study, we describe a methodology to analyze the C924T genotype. The protocol consists of three phases: DNA extraction, amplification by polymerase chain reaction (PCR), and analysis of the restriction fragment length polymorphism (RFLP) on agarose gel.
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De Iuliis, V., Ursi, S., Pennelli, A., Caruso, M., Capodifoglio, S., Marino, A., Flati, V., Vitullo, G., Toniato, E., Robuffo, I., Martinotti, S. A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene. J. Vis. Exp. (146), e57289, doi:10.3791/57289 (2019).
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