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In Vivo Fiber-Coupled Pre-Clinical Confocal Laser-scanning Endomicroscopy (pCLE) of Hippocampal Capillaries in Awake Mice
Chapters
- 00:05Introduction
- 01:18Stereotaxic Positioning
- 03:27Head-Cap Installation
- 04:33Imaging Window
- 05:55In Vivo Fiber-Optic-Bundle-Coupled Pre-Clinical Confocal Laser-Scanning Endomicroscopy (pCLE)
- 07:27Results: Representative Mural Cell Vasoconstriction and Recorded Brain Immunohistochemistry
- 08:26Conclusion
Whereas multiphoton imaging is only effective at limited depths from the tissue’s surface, it is possible to achieve 3 µm resolution imaging at any depth via pCLE. Here, we present a protocol to conduct pCLE imaging to measure microvascular dynamics in the hippocampus of ictal and wild-type mice.
