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In Vivo Fiber-Coupled Pre-Clinical Confocal Laser-scanning Endomicroscopy (pCLE) of Hippocampal Capillaries in Awake Mice
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
In Vivo Fiber-Coupled Pre-Clinical Confocal Laser-scanning Endomicroscopy (pCLE) of Hippocampal Capillaries in Awake Mice

In Vivo Fiber-Coupled Pre-Clinical Confocal Laser-scanning Endomicroscopy (pCLE) of Hippocampal Capillaries in Awake Mice

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09:08 min

April 21, 2023

DOI:

09:08 min
April 21, 2023

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Summary

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Whereas multiphoton imaging is only effective at limited depths from the tissue’s surface, it is possible to achieve 3 µm resolution imaging at any depth via pCLE. Here, we present a protocol to conduct pCLE imaging to measure microvascular dynamics in the hippocampus of ictal and wild-type mice.

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