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Pax7 和层粘连蛋白抗体免疫荧光法鉴定骨骼肌卫星细胞
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Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies
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Pax7 和层粘连蛋白抗体免疫荧光法鉴定骨骼肌卫星细胞

DOI:
10.3791/57212-v

•

07:18 min

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April 19, 2018

•

, , , ,
1Laboratory of Muscle Stem Cells and Gene Regulation,National Institutes of Health, 2Office of Science and Technology, National Institute of Arthritis, Musculoskeletal and Skin Diseases (NIAMS),National Institutes of Health

Chapters

  • 00:05Title
  • 00:41Cryo-embedding of the Tibialis Anterior Muscle
  • 01:46Cryostat Sectioning
  • 02:33Immunofluorescence and Staining
  • 05:27Results: Visualization of Satellite Cells in Adult Resting Muscle Sections is Improved Using Immunofluorescence-based Staining
  • 06:40Conclusion

Summary

자동 번역

자동 번역

卫星细胞的精确识别对于研究其在各种生理和病理条件下的功能至关重要。本文提出了一种基于免疫荧光染色的成人骨骼肌切片卫星细胞识别的协议。

Tags

Skeletal MuscleSatellite CellsImmunofluorescencePax7LamininCryosectioningCryostatAntigen RetrievalImmunostaining

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