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Inmunoprecipitación de cromatina (ChIP) de modificaciones de las histonas de Saccharomyces cerevisiae
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Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae
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Inmunoprecipitación de cromatina (ChIP) de modificaciones de las histonas de Saccharomyces cerevisiae

12,469 Views

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11:06 min

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December 29, 2017

DOI:

10.3791/57080-v

DOI:
10.3791/57080-v

•

11:06 min
December 29, 2017

•

12 Views
, , ,
1Department of Biological Sciences,University of Maryland

Summary

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Aquí, describimos un protocolo para la inmunoprecipitación de cromatina de histonas modificadas de la levadura de florecimiento Saccharomyces cerevisiae. Immunoprecipitated ADN se utiliza posteriormente para PCR cuantitativa para interrogar la abundancia y localización de modificaciones post-traduccionales de las histonas por todo el genoma.

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Jezek, M., Jacques, A., Jaiswal, D., Green, E. M. Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae. J. Vis. Exp. (130), e57080, doi:10.3791/57080 (2017).

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