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면역학 및 감염병학

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体内人淋巴组织和女性生殖器粘膜与人体免疫机能丧失病毒1和 Histoculture 的感染

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture

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体内人淋巴组织和女性生殖器粘膜与人体免疫机能丧失病毒1和 Histoculture 的感染

DOI:

10.3791/57013-v

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11:14 min

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October 12, 2018

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Andrea Introini², Christophe Vanpouille, Wendy Fitzgerald, Kristina Broliden, Leonid Margolis

¹Department of Medicine Solna, Center for Molecular Medicine, Karolinska University Hospital,Karolinska Institutet, ²Section of Intercellular Interactions, Eunice Shriver National Institute of Child Health and Human Development,National Institutes of Health

Chapters

00:04Title
00:49Preparation of Gelatin Sponges
01:55Dissection and Infection of Tonsillar Tissue with HIV-1
04:13Dissection and Infection of Uterine Cervical Mucosa with HIV-1
07:17Histoculture
08:33Results: Kinetics of HIV-1 Replication in Cervical Mucosa Explants
10:02Conclusion

Summary

자동 번역

人类组织感染人体免疫机能丧失病毒 (HIV) 的体内提供了一个有价值的3D 模型的病毒发病机制。在这里, 我们描述了处理和感染人体扁桃体和女性生殖器粘膜与 HIV-1 的组织标本的协议, 并在液体-空气界面保持他们在文化。

Tags

Keywords: Ex Vivo HIV Female Genital Mucosa Virus Transmission Pathogenesis HIV Infection Tonsils Cervix CMT Medium Gelatin Sponges

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