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Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture
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JoVE 杂志 免疫与感染
Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture

Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture

DOI:
10.3791/57013-v

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11:14 min

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October 12, 2018

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, , , ,
1Department of Medicine Solna, Center for Molecular Medicine, Karolinska University Hospital,Karolinska Institutet, 2Section of Intercellular Interactions, Eunice Shriver National Institute of Child Health and Human Development,National Institutes of Health

Chapters

  • 00:04Title
  • 00:49Preparation of Gelatin Sponges
  • 01:55Dissection and Infection of Tonsillar Tissue with HIV-1
  • 04:13Dissection and Infection of Uterine Cervical Mucosa with HIV-1
  • 07:17Histoculture
  • 08:33Results: Kinetics of HIV-1 Replication in Cervical Mucosa Explants
  • 10:02Conclusion

Summary

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Infection of human tissues with human immunodeficiency virus (HIV) ex vivo provides a valuable 3D model of virus pathogenesis. Here, we describe a protocol to process and infect tissue specimens from human tonsils and female genital mucosae with HIV-1 and maintain them in culture at the liquid-air interface.

Tags

Ex VivoHIVFemale Genital MucosaVirus TransmissionPathogenesisHIV InfectionTonsilsCervixCMT MediumGelatin Sponges

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