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Imunologia e Infecção
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Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture

Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture

8,907 Views

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11:14 min

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October 12, 2018

DOI:

10.3791/57013-v

DOI:
10.3791/57013-v

•

11:14 min
October 12, 2018

•

2 Views
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1Department of Medicine Solna, Center for Molecular Medicine, Karolinska University Hospital,Karolinska Institutet, 2Section of Intercellular Interactions, Eunice Shriver National Institute of Child Health and Human Development,National Institutes of Health

Summary

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Infection of human tissues with human immunodeficiency virus (HIV) ex vivo provides a valuable 3D model of virus pathogenesis. Here, we describe a protocol to process and infect tissue specimens from human tonsils and female genital mucosae with HIV-1 and maintain them in culture at the liquid-air interface.

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Cite this Article

Introini, A., Vanpouille, C., Fitzgerald, W., Broliden, K., Margolis, L. Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture. J. Vis. Exp. (140), e57013, doi:10.3791/57013 (2018).

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