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Laminaire stroming gebaseerde testen te onderzoeken Leukocyte aanwerving op gekweekte vasculaire cellen en aanhangend bloedplaatjes
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
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Laminaire stroming gebaseerde testen te onderzoeken Leukocyte aanwerving op gekweekte vasculaire cellen en aanhangend bloedplaatjes

7,435 Views

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08:50 min

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April 09, 2018

DOI:

10.3791/57009-v

DOI:
10.3791/57009-v

•

08:50 min
April 09, 2018

•

6 Views
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1Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM),Maastricht University, 2Department of Vascular Surgery, Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University, School of Medicine

Summary

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Leukocyten interactie gretig met vasculaire cellen en trombocyten na vaartuig muur letsel of tijdens ontsteking. Hier beschrijven we een eenvoudig laminaire flow gebaseerde assay karakteriseren de moleculaire mechanismen die ten grondslag liggen aan de interacties tussen leukocyten en hun cellulaire partners.

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Keyword Extraction:Leukocyte RecruitmentAdherent Vascular CellsAdherent PlateletsFlow ConditionsLaminar Flow-based AssaysImmunologyLeukocyte AdhesionTumor Cell AdhesionPlatelet-rich PlasmaPlatelet-poor PlasmaPlatelet BufferHydrochloric Acid EthanolRat Collagen Type OnePhosphate Buffered Saline

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Vajen, T., Heinzmann, A. C., Dickhout, A., Zhao, Z., Nagy, M., Heemskerk, J. W., Koenen, R. R. Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets. J. Vis. Exp. (134), e57009, doi:10.3791/57009 (2018).

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