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以层流为基础的检测培养血管细胞和粘附血小板白细胞的研究
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JoVE 신문 면역학 및 감염병학
Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
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以层流为基础的检测培养血管细胞和粘附血小板白细胞的研究

7,435 Views

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08:50 min

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April 09, 2018

DOI:

10.3791/57009-v

DOI:
10.3791/57009-v

•

08:50 min
April 09, 2018

•

6 Views
, , , , , ,
1Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM),Maastricht University, 2Department of Vascular Surgery, Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University, School of Medicine

Summary

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在血管壁损伤或炎症期间, 白细胞与血管细胞和血小板相互作用。在这里, 我们描述了一种简单的基于层流的检测方法来表征白细胞与其细胞伙伴之间相互作用的分子机制。

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Keyword Extraction:Leukocyte RecruitmentAdherent Vascular CellsAdherent PlateletsFlow ConditionsLaminar Flow-based AssaysImmunologyLeukocyte AdhesionTumor Cell AdhesionPlatelet-rich PlasmaPlatelet-poor PlasmaPlatelet BufferHydrochloric Acid EthanolRat Collagen Type OnePhosphate Buffered Saline

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Vajen, T., Heinzmann, A. C., Dickhout, A., Zhao, Z., Nagy, M., Heemskerk, J. W., Koenen, R. R. Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets. J. Vis. Exp. (134), e57009, doi:10.3791/57009 (2018).

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