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Wasmiddel-vrije ultrasnelle reconstitutie van membraaneiwitten in lipide-Bilayers met behulp van Fusogenic aanvullende geladen Proteoliposomes.
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생화학
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JoVE 신문 생화학
Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.
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Wasmiddel-vrije ultrasnelle reconstitutie van membraaneiwitten in lipide-Bilayers met behulp van Fusogenic aanvullende geladen Proteoliposomes.

10,777 Views

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11:10 min

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April 05, 2018

DOI:

10.3791/56909-v

DOI:
10.3791/56909-v

•

11:10 min
April 05, 2018

•

4 Views
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1First Pavlov State Medical University, 2Clarendon Laboratory, Department of Physics,Oxford University, 3Department of Biological Sciences,Southern Methodist University

Summary

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Hier presenteren we twee ultrasnelle protocollen voor reconstructie van membraaneiwitten in fusogenic proteoliposomes, en de fusie van dergelijke proteoliposomes met doel lipide bilayers voor wasmiddel-gratis bezorging van deze membraaneiwitten binnen de postfusion dubbelgelaagde. De combinatie van deze benaderingen kan snel en gemakkelijk-gecontroleerde vergadering van complexe multi-component membraan systemen.

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Membrane ProteinsLipid BilayersFusogenic ProteoliposomesDetergent-freeReconstitutionArtificial CellMembrane TransportSynthetic Biology

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Galkin, M. A., Russell, A. N., Vik, S. B., Berry, R. M., Ishmukhametov, R. R. Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.. J. Vis. Exp. (134), e56909, doi:10.3791/56909 (2018).

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