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Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.
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Biochemie
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JoVE Journal Biochemie
Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.

Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.

10,857 Views

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11:10 min

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April 05, 2018

DOI:

10.3791/56909-v

DOI:
10.3791/56909-v

•

11:10 min
April 05, 2018

•

4 Views
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1First Pavlov State Medical University, 2Clarendon Laboratory, Department of Physics,Oxford University, 3Department of Biological Sciences,Southern Methodist University

Summary

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Here we present two ultrafast protocols for reconstitution of membrane proteins into fusogenic proteoliposomes, and fusion of such proteoliposomes with target lipid bilayers for detergent-free delivery of these membrane proteins into the postfusion bilayer. The combination of these approaches enables fast and easily controlled assembly of complex multi-component membrane systems.

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Membrane ProteinsLipid BilayersFusogenic ProteoliposomesDetergent-freeReconstitutionArtificial CellMembrane TransportSynthetic Biology

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Galkin, M. A., Russell, A. N., Vik, S. B., Berry, R. M., Ishmukhametov, R. R. Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.. J. Vis. Exp. (134), e56909, doi:10.3791/56909 (2018).

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