/
/
Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.
This content is Free Access.
Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.
10,857 Views
•
11:10 min
•
April 05, 2018
DOI:
Summary
Automatically generated
Here we present two ultrafast protocols for reconstitution of membrane proteins into fusogenic proteoliposomes, and fusion of such proteoliposomes with target lipid bilayers for detergent-free delivery of these membrane proteins into the postfusion bilayer. The combination of these approaches enables fast and easily controlled assembly of complex multi-component membrane systems.
Read Article
Cite this Article
Galkin, M. A., Russell, A. N., Vik, S. B., Berry, R. M., Ishmukhametov, R. R. Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.. J. Vis. Exp. (134), e56909, doi:10.3791/56909 (2018).
Copy