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小鼠膈运动神经元逆行神经解剖学追踪的研究

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Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice

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小鼠膈运动神经元逆行神经解剖学追踪的研究

DOI:

10.3791/56758-v

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09:05 min

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February 22, 2018

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Jean-Michel Vandeweerd, Fanny Hontoir, Alexis De Knoop, Kathleen De Swert, Charles Nicaise

¹URVI, NARILIS,Université de Namur, ²URPhyM, NARILIS,Université de Namur

Chapters

00:05Title
01:23Preparations
02:22Intrapleural Injections Using Transdiaphragmatic Approach
04:48Intrapleural Injection using Transthoracic Approach and Post-operative Care
06:38Results: Labeling Neurons with CTB after a C4 Spinal Contusion
08:01Conclusion

Summary

자동 번역

本文描述了荧光共轭霍乱毒素亚单位β胸腔内分娩后小鼠膈运动神经元识别的一种协议。比较两种方法注射胸腔: 膈与经胸的方法。

Tags

Keywords: Retrograde Neuroanatomical Tracing Phrenic Motor Neurons Mice Intrapleural Delivery Fluorophore-conjugated Cholera Toxin Subunit Beta Transdiaphragmatic Approach Transthoracic Approach Neuromuscular Junctions Pleural Space Sterile Surgery Anesthesia Skin Preparation CTB Solution Microsyringe Laparotomy

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