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Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers
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Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers
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11:34 min
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December 05, 2017
DOI:
1Division of Pathology,Fondazione IRCCS Ca’ Granda, Ospedale Maggiore Policlinico, 2School of Medicine,University of Milan, 3School of Biology,University of Pavia, 4Division of Breast Surgery,Fondazione IRCCS Ca’ Granda – Ospedale Maggiore Policlinico, 5Division of Medical Oncology,Fondazione IRCCS Ca’ Granda – Ospedale Maggiore Policlinico, 6Department of Biomedical, Surgical, and Dental Sciences,University of Milan
Summary
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Heterogeneous distribution of HER2-positive cells can be observed in a subset of breast cancers and generates clinical dilemmas. Here, we introduce a reliable and cost-effective protocol to define, quantify, and compare HER2 intra-tumor genetic heterogeneity in a large series of heterogeneously processed breast cancers.
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Ercoli, G., Lopez, G., Ciapponi, C., Corti, C., Despini, L., Gambini, D., Runza, L., Blundo, C., Sciarra, A., Fusco, N. Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers. J. Vis. Exp. (130), e56686, doi:10.3791/56686 (2017).
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