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Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers
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Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers
1Division of Pathology,Fondazione IRCCS Ca’ Granda, Ospedale Maggiore Policlinico, 2School of Medicine,University of Milan, 3School of Biology,University of Pavia, 4Division of Breast Surgery,Fondazione IRCCS Ca’ Granda – Ospedale Maggiore Policlinico, 5Division of Medical Oncology,Fondazione IRCCS Ca’ Granda – Ospedale Maggiore Policlinico, 6Department of Biomedical, Surgical, and Dental Sciences,University of Milan
Capítulos
- 00:05Título
- 01:24Design and Construct Tissue Microarrays (TMAs) Based on the Kononen Technique
- 06:20Silver In Situ Hybridization (SISH) Analysis of Human Epidermal Growth Factor Receptor 2 (HER2)
- 09:11Results: Analysis of Heterogeneity of HER2 Gene Amplification in Breast Cancers
- 10:00Conclusion
Heterogeneous distribution of HER2-positive cells can be observed in a subset of breast cancers and generates clinical dilemmas. Here, we introduce a reliable and cost-effective protocol to define, quantify, and compare HER2 intra-tumor genetic heterogeneity in a large series of heterogeneously processed breast cancers.
