Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / A Simple Cell-based Immunofluorescence Assay to…
JoVE Journal
Neuroscience

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE Journal Neuroscience
A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood
 

A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood

Article DOI: 10.3791/56676-v • 07:20 min • January 9th, 2018
January 9th, 2018 •
1,2, 1
1Department of Psychiatry, Chang Gung Memorial Hospital-Linkou, 2Department and Graduate Institute of Biomedical Sciences, Chang Gung University

챕터

요약
Automatic Translation

We ectopically expressed NR1 subunit of NMDA receptor tagged with green fluorescent protein in human embryonic cells (HEK293) as antigen to detect autoantibodies against NMDA receptor in the blood of patients suspected with autoimmune encephalitis. This simple method may be suitable for screening purposes in clinical settings.

Tags

Keywords: Cell-based Immunofluorescence Assay NMDA Receptor Autoantibody Autoimmune Encephalitis HEK293 Cells NR1-GFP Plasmid Transfection Fluorescence Microscopy Paraformaldehyde PBST Skim Milk
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter