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分離とシロイヌナズナ由来のミトコンドリアの呼吸測定
JoVE 신문
생화학
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JoVE 신문 생화학
Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana
DOI:

09:54 min

January 05, 2018

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Chapters

  • 00:05Title
  • 00:50Preparation of Grinding Medium, Wash Buffer, and Gradient Solutions
  • 02:22Homogenization and Mitochondrial Isolation
  • 06:30Oxygen Consumption Measuurements
  • 08:42Results: Isolation of Purified Mitochondrial Protein and Oxygen Consumption Measurement of Purified Mitochondria Obtained from Arabidopsis thaliana Plants
  • 09:37Conclusion

Summary

자동 번역

ミトコンドリアは、植物細胞のわずかな割合は、彼ら研究の範囲を精製する必要があります。ミトコンドリアは、均質化、続いて高純度ミトコンドリア分画を取得する微分と密度勾配遠心法によってさまざまな植物器官から分離できます。

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