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Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana
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JoVE Journal Biochimica
Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana

15,063 Views

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09:54 min

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January 05, 2018

DOI:

10.3791/56627-v

DOI:
10.3791/56627-v

•

09:54 min
January 05, 2018

•

14 Views
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1ARC Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science,La Trobe University, 2School of Biological Sciences,Flinders University, 3ARC Centre of Excellence in Plant Energy Biology,University of Western Australia

Summary

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As mitochondria are only a small percentage of the plant cell, they need to be purified for a range of studies. Mitochondria can be isolated from a variety of plant organs by homogenization, followed by differential and density gradient centrifugation to obtain a highly purified mitochondrial fraction.

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Cite this Article

Lyu, W., Selinski, J., Li, L., Day, D. A., Murcha, M. W., Whelan, J., Wang, Y. Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana. J. Vis. Exp. (131), e56627, doi:10.3791/56627 (2018).

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