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Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos
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Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

9,375 Views

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09:42 min

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October 12, 2017

DOI:

10.3791/56446-v

DOI:
10.3791/56446-v

•

09:42 min
October 12, 2017

•

3 Views
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1Cell, Developmental, and Regenerative Biology Department,Icahn School of Medicine at Mount Sinai, 2Mindich Child Health and Development Institute,Icahn School of Medicine at Mount Sinai, 3Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai, 4Microscopy Core,Icahn School of Medicine at Mount Sinai, 5Department of Neuroscience,Icahn School of Medicine at Mount Sinai, 6Friedman Brain Institute,Icahn School of Medicine at Mount Sinai

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Quantitative Whole-mount ImmunofluorescenceCardiac Progenitor PopulationsMouse EmbryosHeart DevelopmentEmbryonic OrganogenesisVolumetric ImagingAutomated AnalysisEarly EmbryogenesisOrgan MorphogenesisEmbryo DissectionParaformaldehyde FixationImmunofluorescence Staining

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Bardot, E., Tzavaras, N., Benson, D. L., Dubois, N. C. Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos. J. Vis. Exp. (128), e56446, doi:10.3791/56446 (2017).

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