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Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos
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Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos
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October 12, 2017
DOI:
1Cell, Developmental, and Regenerative Biology Department,Icahn School of Medicine at Mount Sinai, 2Mindich Child Health and Development Institute,Icahn School of Medicine at Mount Sinai, 3Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai, 4Microscopy Core,Icahn School of Medicine at Mount Sinai, 5Nörobilim Bölümü,Icahn School of Medicine at Mount Sinai, 6Friedman Brain Institute,Icahn School of Medicine at Mount Sinai
Özet
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Here, we describe a protocol for whole mount immunofluorescence and image-based quantitative volumetric analysis of early stage mouse embryos. We present this technique as a powerful approach to qualitatively and quantitatively assess cardiac structures during development, and propose that it may be widely adaptable to other organ systems.
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Bardot, E., Tzavaras, N., Benson, D. L., Dubois, N. C. Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos. J. Vis. Exp. (128), e56446, doi:10.3791/56446 (2017).
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