Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Rosa G&#243;mez-Villafuertes; Luc&#237;a Paniagua-Herranz; Sergio Gascon; David de Agust&#237;n-Dur&#225;n; Mar&#237;a de la O Ferreras; Juan Carlos Gil-Redondo; Mar&#237;a Jos&#233; Queipo; Aida Menendez-Mendez; R&#225;quel P&#233;rez-Sen; Esmerilda G. Delicado; Javier Gualix; Marcos R. Costa; Timm Schroeder; Mar&#237;a Teresa Miras-Portugal; Felipe Ortega

doi:10.3791/56291

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Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

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Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

DOI:

10.3791/56291-v

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10:55 min

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December 16, 2017

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Rosa Gómez-Villafuertes*^1,2,3, Lucía Paniagua-Herranz*^1,2,3, Sergio Gascon*^4,5, David de Agustín-Durán^2,3, María de la O Ferreras^2,3, Juan Carlos Gil-Redondo^2,3, María José Queipo^2,3, Aida Menendez-Mendez^2,3, Ráquel Pérez-Sen^2,3, Esmerilda G. Delicado^2,3, Javier Gualix^2,3, Marcos R. Costa, Timm Schroeder, María Teresa Miras-Portugal^2,3, Felipe Ortega^2,3

¹Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine,Complutense University, ²University Institute for Neurochemistry Research (IUIN), ³Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), ⁴Institute of Stem Cell Research, Helmholtz Center Munich, Neuherberg/Munich, Germany Physiological Genomics, Biomedical Center,Ludwig-Maximilians University Munich, ⁵Toxicology and Pharmacology Department, Faculty of Veterinary medicine,Complutense University, ⁶Brain Institute,Federal University of Rio Grande do Norte, ⁷Department of Biosystems Science and Engineering,Eidgenössische Technische Hochschule (ETH) Zurich

Chapters

00:05Title
01:14Live Imaging by Time-lapse Video-microscopy
04:02Post-imaging Immunocytochemistry, Data Collection, and Processing
06:38Single-cell Tracking
08:56Results: Example of aNSCs Isolated from the SEZ and Analyzed by Live Imaging and Single Cell Tracking
10:17Conclusion

Summary

자동 번역

A robust protocol to monitor neural populations by time-lapse video-microscopy followed by software-based post-processing is described. This method represents a powerful tool to identify biological events in a selected population during live imaging experiments.

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

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자동 번역

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