Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Rosa G&#243;mez-Villafuertes; Luc&#237;a Paniagua-Herranz; Sergio Gascon; David de Agust&#237;n-Dur&#225;n; Mar&#237;a de la O Ferreras; Juan Carlos Gil-Redondo; Mar&#237;a Jos&#233; Queipo; Aida Menendez-Mendez; R&#225;quel P&#233;rez-Sen; Esmerilda G. Delicado; Javier Gualix; Marcos R. Costa; Timm Schroeder; Mar&#237;a Teresa Miras-Portugal; Felipe Ortega

doi:10.3791/56291

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Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

JoVE Journal
Neurociência

This content is Free Access.

JoVE Journal Neurociência

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

8,307 Views

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10:55 min

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December 16, 2017

DOI:

10.3791/56291-v

DOI:

10.3791/56291-v

•

10:55 min

December 16, 2017

•

5 Views

Rosa Gómez-Villafuertes*^1,2,3, Lucía Paniagua-Herranz*^1,2,3, Sergio Gascon*^4,5, David de Agustín-Durán^1,2,3, María de la O Ferreras^1,2,3, Juan Carlos Gil-Redondo^1,2,3, María José Queipo^1,2,3, Aida Menendez-Mendez^1,2,3, Ráquel Pérez-Sen^1,2,3, Esmerilda G. Delicado^1,2,3, Javier Gualix^1,2,3, Marcos R. Costa⁶, Timm Schroeder⁷, María Teresa Miras-Portugal^1,2,3, Felipe Ortega^1,2,3

¹Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine,Complutense University, ²University Institute for Neurochemistry Research (IUIN), ³Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), ⁴Institute of Stem Cell Research, Helmholtz Center Munich, Neuherberg/Munich, Germany Physiological Genomics, Biomedical Center,Ludwig-Maximilians University Munich, ⁵Toxicology and Pharmacology Department, Faculty of Veterinary medicine,Complutense University, ⁶Brain Institute,Federal University of Rio Grande do Norte, ⁷Department of Biosystems Science and Engineering,Eidgenössische Technische Hochschule (ETH) Zurich

Summary

A robust protocol to monitor neural populations by time-lapse video-microscopy followed by software-based post-processing is described. This method represents a powerful tool to identify biological events in a selected population during live imaging experiments.

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Live Imaging Single Cell Tracking Neural Populations Cell Biology Lineage Progression Immunocytochemistry Cell Behavior Cell Fate Proliferation Differentiation Time-lapse Video-microscopy Cell Population Neural Cell

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Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

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