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Journal / Genetics / Microinjection CRISPR/Cas9 protein i kanalen…
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Genetics
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JoVE Journal Genetics
Microinjection CRISPR/Cas9 protein i kanalen Gråsteinbit, Ictalurus punctatus, embryo for Gene Editing
 
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Microinjection CRISPR/Cas9 protein i kanalen Gråsteinbit, Ictalurus punctatus, embryo for Gene Editing

Article DOI: 10.3791/56275-v • 16:08 min • January 20th, 2018
January 20th, 2018 •
*1,2, *1,3, 1, 4, 4, 5, 5, 1
1School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, 2Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, 3Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, 4Department of Molecular Physiology and Biophysics, Vanderbilt University, 5Life Science Institute, University of Michigan
* These authors contributed equally

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En enkel og effektiv microinjection protokoll for genet redigering i kanalen Gråsteinbit embryoer bruker CRISPR/Cas9 systemet er presentert. I denne protokollen, guide RNAs og Cas9 protein var microinjected i eggeplomme av en celle embryo. Denne protokollen er validert av banke ut to kanalen Gråsteinbit immun-relaterte gener.

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Genetikk problemet 131 CRISPR/Cas9 gene redigering gene knockout microinjection kanalen Gråsteinbit embryo mutasjon avkortet protein indeler
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