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Mikroskop av CRISPR/Cas9 Protein till kanal havskatt, Ictalurus punctatus, embryon för Gene Editing
1School of Fisheries, Aquaculture and Aquatic Sciences,Auburn University, 2Department of Animal Wealth Development, Faculty of Veterinary Medicine,Suez Canal University, 3Anatomy and Embryology Department, Faculty of Veterinary Medicine,Cairo University, 4Department of Molecular Physiology and Biophysics,Vanderbilt University, 5Life Science Institute,University of Michigan
Chapters
- 00:00Title
- 01:14Brood Stock Selection and Spawning
- 04:16Sperm Preparation
- 05:33Egg Collection and Fertilization
- 08:07Needle Pulling and Loading
- 10:20Microinjection of Catfish Embryos
- 12:34Results
- 15:24Conclusion
Ett enkelt och effektivt Mikroskop protokoll för genen redigering i kanal havskatt embryon med hjälp av CRISPR/Cas9 systemet presenteras. I detta protokoll, vägleda RNAs och Cas9 protein var microinjected in äggulan i en cell embryon. Detta protokoll har validerats genom att slå ut två kanal havskatt immunrelaterade gener.
