Journal
/
/
Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells
JoVE 신문
암 연구학
This content is Free Access.
JoVE 신문 암 연구학
Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells

Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells

9,241 Views

08:18 min

September 05, 2017

DOI:

08:18 min
September 05, 2017

5 Views
, ,

Summary

Automatically generated

Confocal fluorescence microscopy and laser micro-irradiation offer tools for inducing DNA damage and monitoring the response of DNA repair proteins in selected sub-nuclear areas. This technique has significantly advanced our knowledge of damage detection, signaling, and recruitment. This manuscript demonstrates these technologies to examine single and double strand break repair.

Read Article