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Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells
JoVE Revista
Investigación sobre el cáncer
This content is Free Access.
JoVE Revista Investigación sobre el cáncer
Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells

Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells

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08:18 min

September 05, 2017

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08:18 min
September 05, 2017

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Summary

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Confocal fluorescence microscopy and laser micro-irradiation offer tools for inducing DNA damage and monitoring the response of DNA repair proteins in selected sub-nuclear areas. This technique has significantly advanced our knowledge of damage detection, signaling, and recruitment. This manuscript demonstrates these technologies to examine single and double strand break repair.

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