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全贴装荧光原位杂交和免疫荧光法在斑马鱼中 Multiciliated 细胞的可视化
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Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
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全贴装荧光原位杂交和免疫荧光法在斑马鱼中 Multiciliated 细胞的可视化

7,781 Views

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09:33 min

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November 18, 2017

DOI:

10.3791/56261-v

DOI:
10.3791/56261-v

•

09:33 min
November 18, 2017

•

2 Views
, , , ,
1Department of Biological Sciences,University of Notre Dame

Summary

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纤毛发育对正常器官的形成至关重要。该协议描述了一种优化的方法来标记和可视化纤毛细胞的斑马鱼。

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Marra, A. N., Ulrich, M., White, A., Springer, M., Wingert, R. A. Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence. J. Vis. Exp. (129), e56261, doi:10.3791/56261 (2017).

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