Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent <em>In Situ</em> Hybridization and Immunofluorescence

Amanda N. Marra; Marisa Ulrich; Audra White; Meghan Springer; Rebecca A. Wingert

doi:10.3791/56261

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Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

JoVE 杂志
发育生物学

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JoVE 杂志发育生物学

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

7,853 Views

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09:33 min

•

November 18, 2017

DOI:

10.3791/56261-v

DOI:

10.3791/56261-v

•

09:33 min

November 18, 2017

•

2 Views

Amanda N. Marra¹, Marisa Ulrich¹, Audra White¹, Meghan Springer¹, Rebecca A. Wingert¹

¹Department of Biological Sciences,University of Notre Dame

Summary

Cilia development is vital to proper organogenesis. This protocol describes an optimized method to label and visualize ciliated cells of the zebrafish.

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Multiciliated Cells Zebrafish Whole Mount Fluorescent In Situ Hybridization Immunofluorescence 发育生物学 Pronephros Cilia RNA Transcripts Protein Labeling Antibodies Fixation Rehydration Proteinase K Hybridization Solution RNA Probe

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Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Summary

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