JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
AFM and Microrheology in the Zebrafish Embryo Yolk Cell
Chapters
- 00:05Title
- 01:08Atomic Force Microscopy
- 03:54Particle Tracking Microrheology
- 07:02Results: Cortical Tension Measurements and Rheology of the Cortex and Yolk of the Zebrafish Embryo Yolk Cell During Epiboly
- 08:46Conclusion
The lack of tools to measure material properties and tensional parameters in vivo prevents validating their roles during development. We employed atomic force microscopy (AFM) and nanoparticle-tracking to quantify mechanical features on the intact zebrafish embryo yolk cell during epiboly. These methods are reliable and widely applicable avoiding intrusive interventions.
