Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
AFM and Microrheology in the Zebrafish Embryo Yolk Cell
Capítulos
- 00:05Título
- 01:08Atomic Force Microscopy
- 03:54Particle Tracking Microrheology
- 07:02Results: Cortical Tension Measurements and Rheology of the Cortex and Yolk of the Zebrafish Embryo Yolk Cell During Epiboly
- 08:46Conclusion
The lack of tools to measure material properties and tensional parameters in vivo prevents validating their roles during development. We employed atomic force microscopy (AFM) and nanoparticle-tracking to quantify mechanical features on the intact zebrafish embryo yolk cell during epiboly. These methods are reliable and widely applicable avoiding intrusive interventions.
