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High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster
Chapters
- 00:05Title
- 01:08High Fat Diet Preparation
- 02:15High Fat Diet Feeding
- 03:32Weighing the Flies
- 04:22Grinding the Flies
- 05:50Sample Loading and TAG Content Determination
- 06:54Results: Changes in Fat Content after High Fat Diet Feeding
- 07:39Conclusion
This is a high fat diet feeding protocol to induce obesity in Drosophila, a model for understanding fundamental molecular mechanisms implicated in lipotoxicity. It also provides a high throughput triacylglyceride assay for measuring fat accumulation in Drosophila and potentially other (insect) models under various dietary, environmental, genetic or physiological conditions.
