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High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster
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High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster

High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster

10,818 Views

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08:28 min

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September 13, 2017

DOI:

10.3791/56029-v

DOI:
10.3791/56029-v

•

08:28 min
September 13, 2017

•

4 Views
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1Development, Aging and Regeneration Program,Sanford Burnham Prebys Medical Discovery Institute

Summary

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This is a high fat diet feeding protocol to induce obesity in Drosophila, a model for understanding fundamental molecular mechanisms implicated in lipotoxicity. It also provides a high throughput triacylglyceride assay for measuring fat accumulation in Drosophila and potentially other (insect) models under various dietary, environmental, genetic or physiological conditions.

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Diop, S. B., Birse, R. T., Bodmer, R. High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster. J. Vis. Exp. (127), e56029, doi:10.3791/56029 (2017).

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