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Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons
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Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons

Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons

7,995 Views

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07:31 min

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September 07, 2017

DOI:

10.3791/56026-v

DOI:
10.3791/56026-v

•

07:31 min
September 07, 2017

•

2 Views
, , ,
1Department of Biology and Biotechnology,Worcester Polytechnic Institute, 2Department of Biomedical Engineering,Worcester Polytechnic Institute

Summary

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The use of an adapted "olfactory chip" for the efficient calcium imaging of C. elegans males is described here. Studies of male exposure to glycerol and a pheromone are also shown.

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MicrofluidicOlfactoryC. ElegansPheromonesCalcium TransienceNeuronal ActivitySex-specificNeural CircuitBiogenic PheromonesFluorescinS BasalMicro-fluidic DeviceFlow ControlVacuumGFP FilterNGM Agar Plate

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Cite this Article

Reilly, D. K., Lawler, D. E., Albrecht, D. R., Srinivasan, J. Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons. J. Vis. Exp. (127), e56026, doi:10.3791/56026 (2017).

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