The use of an adapted "olfactory chip" for the efficient calcium imaging of C. elegans males is described here. Studies of male exposure to glycerol and a pheromone are also shown.
Reilly, D. K., Lawler, D. E., Albrecht, D. R., Srinivasan, J. Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons. J. Vis. Exp. (127), e56026, doi:10.3791/56026 (2017).