Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male <em>C. Elegans </em>Head Neurons

Douglas K. Reilly; Daniel E. Lawler; Dirk R. Albrecht; Jagan Srinivasan

doi:10.3791/56026

/

Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons

JoVE Journal
Neuroscienze

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Neuroscienze

Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons

Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons

DOI:

10.3791/56026-v

•

07:31 min

•

September 07, 2017

•

Douglas K. Reilly, Daniel E. Lawler, Dirk R. Albrecht², Jagan Srinivasan

¹Department of Biology and Biotechnology,Worcester Polytechnic Institute, ²Department of Biomedical Engineering,Worcester Polytechnic Institute

Capitoli

00:05Titolo
00:41Microfluidic Device Set Up
02:54Loading C. elegans into the Microfluidic Device
04:44Olfactory Stimulation of C. elegans Using a Microfluidic Device
05:43Results: Neural Response to Chemical Stimuli
06:45Conclusion

Summary

Traduzione automatica

The use of an adapted "olfactory chip" for the efficient calcium imaging of C. elegans males is described here. Studies of male exposure to glycerol and a pheromone are also shown.

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

In vivo Laser Axotomy in C. elegans

A Molecular Readout of Long-term Olfactory Adaptation in C. elegans

Multi-unit Recording Methods to Characterize Neural Activity in the Locust (Schistocerca Americana) Olfactory Circuits

In vivo Neuronal Calcium Imaging in C. elegans

Transplantation of Olfactory Ensheathing Cells to Evaluate Functional Recovery after Peripheral Nerve Injury

Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration

In Vivo Imaging of Dauer-specific Neuronal Remodeling in C. elegans

Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders