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JoVE Journal Developmental Biology
Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos
 

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Article DOI: 10.3791/55629-v • 10:15 min • April 28th, 2017
April 28th, 2017 •
1,2,3, 1,2,3, 1,2,3
1Physical Biology, Buchmann Institute for Molecular Life Sciences (BMLS), 2Cluster of Excellence Frankfurt, Macromolecular Complexes, 3Goethe-Universität Frankfurt am Main – Campus Riedberg

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요약
Automatic Translation

Imaging the morphogenesis of insect embryos with light sheet-based fluorescence microscopy has become state of the art. This protocol outlines and compares three mounting techniques appropriate for Tribolium castaneum embryos, introduces two novel custom-made transgenic lines well suited for live imaging, discusses essential quality controls and indicates current experimental limitations.

Tags

Light Sheet-based Fluorescence Microscopy Tribolium Castaneum Embryos Morphogenesis Cell Migration Proliferation Patterns Photobleaching Phototoxicity Sample Chamber Agarose Column Glass Capillary Distilled Water Embryo Mounting Agarose Steel Cylinder
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