An optimized protocol is presented that enables the depletion of cytoplasmic microtubule organizing centers in mouse oocytes during metaphase I using a near-infrared femtosecond laser.
Londoño-Vásquez, D., Jurkevich, A., Balboula, A. Z. Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes. J. Vis. Exp. (189), e64439, doi:10.3791/64439 (2022).