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Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos
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Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

10,334 Views

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10:15 min

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April 28, 2017

DOI:

10.3791/55629-v

DOI:
10.3791/55629-v

•

10:15 min
April 28, 2017

•

11 Views
, ,
1Physical Biology,Buchmann Institute for Molecular Life Sciences (BMLS), 2Cluster of Excellence Frankfurt,Macromolecular Complexes, 3Goethe-Universität Frankfurt am Main – Campus Riedberg

Summary

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Imaging the morphogenesis of insect embryos with light sheet-based fluorescence microscopy has become state of the art. This protocol outlines and compares three mounting techniques appropriate for Tribolium castaneum embryos, introduces two novel custom-made transgenic lines well suited for live imaging, discusses essential quality controls and indicates current experimental limitations.

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Cite this Article

Strobl, F., Klees, S., Stelzer, E. H. K. Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos. J. Vis. Exp. (122), e55629, doi:10.3791/55629 (2017).

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