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Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads
Chapters
- 00:05Title
- 00:49Basal Glyserol Release and Forskolin (FSK)-stimulated Lipolysis
- 04:36Glycerol Content Determination
- 06:13Results: Triglyceride (TGL) Hydrolysis Generates Free Fatty Acids (FFA) and Glycerol
- 06:45Conclusion
This protocol describes the method of determining basal and forskolin-stimulated lipolysis in inguinal fat pads obtained from normal chow diet (NCD) or high fat diet (HFD) ± capsaicin fed wild type mice. As an index for lipolysis, glycerol release was measured from inguinal adipose fat pads.
