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Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads
JoVE Journal
生化学
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JoVE Journal 生化学
Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads

Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads

DOI:

07:59 min

July 21, 2017

,

  • 00:05標題
  • 00:49Basal Glyserol Release and Forskolin (FSK)-stimulated Lipolysis
  • 04:36Glycerol Content Determination
  • 06:13Results: Triglyceride (TGL) Hydrolysis Generates Free Fatty Acids (FFA) and Glycerol
  • 06:45Conclusion

概要

自動翻訳

This protocol describes the method of determining basal and forskolin-stimulated lipolysis in inguinal fat pads obtained from normal chow diet (NCD) or high fat diet (HFD) ± capsaicin fed wild type mice. As an index for lipolysis, glycerol release was measured from inguinal adipose fat pads.

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