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Voltage-clamp Fluorometry in Xenopus Oocytes Using Fluorescent Unnatural Amino Acids
Chapters
- 00:05Title
- 01:03Oocyte Preparation and Injection
- 04:16VCF Setup
- 05:35VCF Recording
- 07:44Results: Two-color VCF with Anap and TMR
- 08:27Conclusion
This article describes an enhancement of conventional Voltage-Clamp Fluorometry (VCF) where Fluorescent Unnatural Amino Acids (fUAA) are used instead of maleimide dyes, to probe structural rearrangements in ion channels. The procedure includes Xenopus oocyte DNA injection, RNA/fUAA coinjection, and simultaneous current and fluorescence measurements.
