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Voltage-clamp Fluorometry in Xenopus Oocytes Using Fluorescent Unnatural Amino Acids
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Voltage-clamp Fluorometry in Xenopus Oocytes Using Fluorescent Unnatural Amino Acids
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May 27, 2017
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This article describes an enhancement of conventional Voltage-Clamp Fluorometry (VCF) where Fluorescent Unnatural Amino Acids (fUAA) are used instead of maleimide dyes, to probe structural rearrangements in ion channels. The procedure includes Xenopus oocyte DNA injection, RNA/fUAA coinjection, and simultaneous current and fluorescence measurements.
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Kalstrup, T., Blunck, R. Voltage-clamp Fluorometry in Xenopus Oocytes Using Fluorescent Unnatural Amino Acids. J. Vis. Exp. (123), e55598, doi:10.3791/55598 (2017).
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