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A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva

A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva

9,981 Views

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07:35 min

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July 05, 2017

DOI:

10.3791/55591-v

DOI:
10.3791/55591-v

•

07:35 min
July 05, 2017

•

3 Views
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1Lee Kong Chian School of Medicine,Nanyang Technological University, 2Singapore Eye Research Institute, 3Singapore National Eye Center, 4Duke-NUS Medical School, 5Yong Loo Lin School of Medicine

Summary

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The exposed normal ocular surface consists of cornea and conjunctiva. Epithelial cells, goblet cells and immune cells are present in the conjunctiva. Here, a non-invasive, technique of impression cytology is described using an impression cytology device and flow cytometry to analyze immune cells in the conjunctiva.

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Impression CytologyOcular SurfaceImmune CellsNon-invasiveCell PhenotypingOcular InflammationOcular ToxicologyOcular FibrosisFlow CytometryConjunctiva

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Cite this Article

Bose, T., Hou, A., Lee, R., Tong, L., Chandy, K. G. A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva. J. Vis. Exp. (125), e55591, doi:10.3791/55591 (2017).

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