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A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva

A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva

DOI:

07:35 min

July 05, 2017

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Kapitel

  • 00:05Titel
  • 01:14Ocular Cell Impression Cytology Collection and Flow Cytometric Phenotyping
  • 05:48Results: Representative Healthy Human Ocular T Cells Subsets
  • 06:35Conclusion

Summary

Automatische Übersetzung

The exposed normal ocular surface consists of cornea and conjunctiva. Epithelial cells, goblet cells and immune cells are present in the conjunctiva. Here, a non-invasive, technique of impression cytology is described using an impression cytology device and flow cytometry to analyze immune cells in the conjunctiva.

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